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yratuit Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Additionally, it is recommended that payat verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Wash membrane six times for 5 min each in TBST. Biotinylated Protein Ladder Detection Pack: Dilute to 1X with dH2O. Do not let dry.
Wash three times for 5 min each with 15 ml of TBST.